PCR-RFLP TECHNIQUE FOR SPECIES ORIGIN IDENTIFICATION OF IMPORTED BUFFALO MEAT
نویسندگان
چکیده
منابع مشابه
Identification of Meat Species by Polymerase Chain Reaction ( PCR ) Technique
The origin of horse, dog, cat, bovine, sheep, porcine, and goat meat was determined by the polymerase chain reaction (PCR) technique, using species-specific primers. Test mixtures of meat were prepared by adding 5%, 2.5%, 1%, 0.5%, and 0.1% levels of pork, horse, cat, or dog meat to beef, sheep, and goat meat. Samples taken from those combinations were analyzed by PCR for species determination....
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Meat adulterations of different species are undetectable and it is common practice globally. In the field of food analysis, species determination is mostly sufficient, but simultaneous detection of several species in a single food product is desirable. The aim of the study was to distinguish between meats of two different species through PCR-RFLP analysis. The meat of two species were used incl...
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The objective of the study was to genotype Pandharpuri buffalo breeds for k-casein type by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). A 379-bp k-casein gene fragment was amplified using primers K1 (5’CAC GTC ACC CAC ACC CAC ATT TAT C-3’) and K2 (5’-TAA TTA GCC CAT TTC GCC TTC TCT GT-3’). DNA from 20 Pandharpuri buffaloes were extracted by phenol chloroform me...
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ژورنال
عنوان ژورنال: Mesopotamia Journal of Agriculture
سال: 2020
ISSN: 2224-9796
DOI: 10.33899/magrj.2020.128802.1086